Year: 2021 | Month: June | Volume 11 | Issue 3
Screening of Non Tuberculous Mycobacterium Species from Farm Environment
Sangavi Chokkalingam
Porteen Kannan
Sureshkannan Sundaram
B.S. MRonald
DOI:10.30954/2277-940X.03.2021.16
Abstract:
The ubiquitous presence of NTM species in the environment is one factor which leads to misdiagnosis, treatment failure of tuberculosis throughout the world. The aim of the present study was to screen samples from farm environment to detect the occurrence of NTM species and to analyze their antimicrobial resistance pattern. A total of 218 samples of water, soil, milk, faeces and sewage were collected and processed as per standard isolation protocols for NTM species. Further, the isolates were subjected to biochemical characterization and molecular confirmation was done by PCR targeting genus specific primers like 16SrRNA and hsp 65. For species identification, the PCR products were sequenced and assessed the genetic relatedness. Antimicrobial resistance of the isolates was also studied by broth microdilution and disc diffusion method. The present study showed the presence of NTM species from farm settings and the isolates were found to be sensitive to antimycobacterial drugs.
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